INTERFERON METHODS ADN PROTOCOLS:116

A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs. Biological and Clinical Basis for Molecular Studies of InterferonsKatie R. Pang, Jashin J. Wu, David B. Huang, Stephen K. Tyring, and Samuel BaronInterferon Research: A Brief HistoryMyriam S. Kunzi and Paula M. PithaVirus Infection and the Interferon Response: A Global View Through Functional GenomicsMarcus J. Korth, John C. Kash, Jeffrey C. Furlong, and Michael G. KatzeGenomic DNA Affinity Chromatography: A Technique to Isolate Interferon-Inducible DNA Binding FactorsJyothi Kumaran and Eleanor N. FishProtein Engineering of Interferon AlphasRenqiu Hu, Ke-jian Lei, Joseph Bekisz, and Kathryn C. ZoonAssays for the Interferon-Induced Enzyme 2',5' Oligoadenylate SynthetasesSaumendra N. Sarkar, Mitali Pandey, and Ganes C. SenA Convenient and Sensitive Fluorescence Resonance Energy Transfer Assay for RNase L and 2',5' OligoadenylatesChandar S. Thakur, Zan Xu, Zhengfu Wang, Zachary Novince, and Robert H. SilvermanRegulation of Murine Interferon Regulatory Factor Gene Expression in the Central Nervous System Determined by Multiprobe RNase Protection AssayShalina S. Ousman and Iain L. CampbellMitogen-Activated Protein Kinase Pathways in Interferon SignalingAntonella Sassano, Amit Verma, and Leonidas C. PlataniasDevelopment of an Interferon-Based Cancer Vaccine Protocol: Application to Several Types of Murine CancersW. Robert Fleischmann, Jr. and Tzu G. WuType I Interferons as Regulators of the Differentiation/Activation of Human Dendritic Cells: Methods for the Evaluation of IFN-Induced EffectsStefano M. Santini, Caterina Lapenta, and Filippo BelardelliFlow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed PopulationsStacey L. Olshalsky and Patricia Fitzgerald-BocarslyAnalysis of Anti-Interferon Properties of the Herpes Simplex Virus Type IICP0 ProteinKaren MossmanInterferon Subtype Gene Therapy for Regulating Cytomegalovirus DiseaseCassandra M. James, Emmalene J. Bartlett, Josephine P. Mansfield, and Vanessa S. CullTransfection of Muller Cells With Type I Interferon Transgenes: Resistance to HSV-1 InfectionBenitta John-Philip and Daniel J. J. CarrIndex